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Trendalert – CRISPR-cas9 Targeted Enrichment Metagenomics
15 augustus 2025
Ensuring safe (drinking) water requires the ability to detect pathogens, virulence genes, antimicrobial resistance genes (ARGs), and indicator organisms even at very low concentrations. However, commonly used molecular methods, such as 16S rRNA gene sequencing, metagenomics, and metatranscriptomics, often lack the sensitivity needed to identify rare or low-abundance targets, especially in low-biomass environments such as drinking water.
CRISPR-based targeted metagenomics offers a solution by enabling precise selection, enrichment, and sequencing of specific low-abundant DNA or RNA from microorganisms of interest.
CRISPR-Cas9-enriched metagenomics uses the Cas9 protein, guided by a custom-designed RNA, to cut out only the DNA sequences of interest from a complex mix. This “enriches” the amount of target DNA in the sample, making it easier to study specific microbes or genes.
Studies using CRISPR-Cas9 targeted enrichment metagenomics have successfully detected low-abundance microorganisms.
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